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doi: 10.3390/vaccines4040046 66 CassimSPouyssegurJ

inflammation of the vein used for insertion

On-target deletion mutations were around the start codon of RSMC

Four cysteine (Cys) are introduced to form disulfide bonds for scFv stability

Abbreviations AMP: adenosine monophosphate AMPK: AMP-activated protein kinase ATF4: activating transcription factor 4 ATG : autophagy-related genes Atg: autophagy-related proteins ATP: adenosine triphosphate Bcl-2: B-cell lymphoma 2 Bcl-x L : Bcl-2 homolog X long protein : BECN1 gene encoding Beclin1 BNIP3: Bcl-2/adenovirus E1B 19 kDa interacting protein 3 Caspase: cysteinyl aspartate-specific proteinase CHOP: C/EBP homologous protein CoCl 2 : cobalt chloride CQ: chloroquine EGFR: epidermal growth factor receptor ER: endoplasmic reticulum FoxO3: forkhead box O3 GBM: glioblastoma multiforme GLUT1,3: glucose transporter-1,3 GTP: guanosine triphosphate HCQ: hydroxychloroquine HIF1: hypoxia-inducible factor 1 IDH: isocitrate dehydrogenase LC3: light chain 3 3-MA: 3-methyladenine mTOR: mammalian target of rapamycin mTORC: mTOR complex O 2 : dioxygen p16InK4a: p16 cyclin-dependent kinase inhibitor 4a PI3K: phosphatidyl-inositide 3 kinase PML: promyelocytic leukemia PTEN: phosphatase and tensin REDD1: regulated in development and DNA damage responses 1 RHEB: Ras homolog enriched in brain shRNA: small hairpin RNA siRNA: small interfering RNA TP53 : gene encoding p53 TSC1/TSC2: tuberous sclerosis complex 1 and 2 ULK1: Unc-51-like autophagy-activating kinase 1 UPR: unfolded protein response UVRAG: UV radiation resistance-associated gene protein VEGF: vascular endothelial growth factor WHO: World Health Organization References Louis DN, Ohgaki H, Wiestler OD, Cavenee WK, Burger PC, Jouvet A et al