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First, all possible deamidations of N/Q to D/E are simulated up to the number detected by search software and ignoring N-terminal Q if the pyroGlu modification was detected, next reverse deamidation permutations of D/E to N/Q are simulated, ignoring previously altered positions and N-terminal E if the pyroGlu modification was detected, and then if N-term pyroGlu formation was detected, Q to E or E to Q substitutions are simulated depending on starting amino acid

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