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Total mRNA isolation, cDNA synthesis, and qPCR Cells were treated with various concentrations of thiotert for 6 h, and total mRNA was extracted by RNA-Quick Purification Kit (Accurate Biology, China) according to the manufacturers instructions

Glutamine acts as a nitrogen donor, which is critical for the synthesis of nucleotides, amino sugars, and other amino acids

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Depending upon the frequency of the genetic defect, a rare event caused by genetic susceptibility could be missed even in large study samples