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Ganoderma lucidum polysaccharides ameliorates hepatic steatosis and oxidative stress in db/db mice via targeting nuclear factor E2 (Erythroid-Derived 2)-Related Factor-2/Heme Oxygenase-1 (HO-1) pathway

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SIRT1 expression is refractory to hypoxia and inflammatory cytokines in nucleus pulposus cells: novel regulation by HIF-1 and NF-B signaling

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Briefly, independent cultures of Agrobacterium carrying pTRV1 or pTRV2-based constructs were resuspended in 10 mM MgCl 2 , 10 mM MES (pH 5.6) and 150 M acetosyringone at OD 600 = 0.8, and incubated for 2 h at 28 C in the dark