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For a 10mg vial of Melanotan-2, the most common reconstitution volumes are 1mL (yielding 10mg/mL) or 2mL (yielding 5mg/mL)

As detailed in the proposed rule, this was based on dividing the standard clinical labor times by 2.25 to account for the MPPR, such as taking the standard 3 minutes and dividing it by 2.25 to arrive at the proposed time of 1.33 minutes (1.33 + 0.67 + 0.67 + 0.34 = 3 minutes)

The cells were imaged with a ZOE Fluorescent Cell Imager (Hercules, USA) and images were analyzed using ImageJ (National Institute of Health, USA)

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doi: 10.1002/path.5221 7 LiuCXiaoKXieL