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GGT activity assay Cell cultures were washed and scraped with PBS

To determine the amount of NFT required after its initial addition and a washout phase to maintain elevated glutathione levels, cells were pretreated with 20 M NFT for 48 h, maintained in NFT-free medium for 24 h, and subsequently treated with varying concentrations of NFT over 24 h

doi: 10.3389/fmolb.2025.1638042 Received 02 June 2025 Accepted 11 August 2025 Published 04 September 2025 Volume 12 - 2025 Edited by Lidija Milkovic, Rudjer Boskovic Institute, Croatia Reviewed by Sathish Sivaprakasam, Texas Tech University Health Sciences Center, United States Jos Daz-Chvez, Instituto Nacional de Cancerologa (INCAN), Mexico Updates Copyright 2025 Gao, Jiang, Sun, An, Sun, Tang, Kang, Zhao, Li, Liu, Ji and Lian

Consult relevant safety data sheets and adhere to laboratory protocols