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doi: 10.1038/ijo.2008.202 46 ManciniADi SegniC

Mammalian cell procedures U2OS cells were cultured in Eagles minimal essential medium (EMEM, Quality Biological) supplemented with 10% fetal bovine serum (Gibco), 2 mM L-glutamine (Gibco), and 10 U mL 1 penicillin-streptomycin (Gibco)

An efficient holistic treatment for this particular symptom proved to be B12 vitamin injections

Includes controls Normalized melting curves illustrating positive control (red), the assay calibration control (blue), and negative control (orange) of GSTP1 templates supplied with the MethylDetect GSTP1 kit