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Insulin-like growth factor-1 (IGF-1), a member of a family of growth factors that are structurally closely related to pro-insulin, has shown profound effects on chondrocyte biological behavior and fundamentally regulates cartilage matrix metabolism during cartilage repair

BMB Rep (2019) 52(6):36072

Specifically, the loss of glutaryl-CoA dehydrogenase leads to intracellular accumulation of glutaryl-CoA, promoting NRF2 glutarylation

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GST pull-down was mixed with cell lysates from HEK293T cells, washed, and immunoblotted for NMHC-IIA and GST