glutathione s transferase purification GST-Tagged Protein Visualization of GST fusions during

So people at greatest risk for deficiency include those with low stomach acid (e.g., older adults and people on proton pump inhibitors or antacids) or low IF production (known as pernicious anemia), people on medications that interfere with B12 absorption (such as metformin), people who have had gastric surgery, those with GI conditions that impair nutrient absorption (like celiac or Crohn's disease), and people on vegan and vegetarian diets

doi: 10.1023/A:1019915306581

Yang P, Ma J, Zhang B, Duan H, He Z, Zeng J, Zeng X, Li D, Wang Q, Xiao Y, Liu C, Xiao Q, Chen L, Zhu X, Xing X, Li Z, Zhang S, Zhang Z, Ma L, Wang E, Zhuang Z, Zheng Y, Chen W

AMPK is known to be activated by H 2 O 2 , probably via oxidation of cysteine 299 and cysteine 304 since mutation of these residues to alanine abrogated the enhancing effect of H 2 O 2 on kinase activity [125]

L-Glutamine provides a source of carbons for oxidation in some cells