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This positions GHK-Cu as a model substrate for studying small-peptide control of large gene-network programs.[9] Redox-Silenced Copper Delivery Studies Bioinorganic chemistry investigations use GHK-Cu as a model carrier for delivering Cu(II) to cells without triggering Fenton-driven hydroxyl-radical generation

Cells were cultured and treated in a 37 C humidified incubator gassed with 5% CO 2 and 90% N 2 so as to achieve bone marrow level hypoxia of 5% O 2 or alternatively room air without N 2 to achieve 20% O 2

J Biol Chem 2008;283:31203129

We cover dietary approaches, probiotics, FMT, and pharmacological agents, evaluating both their biological plausibility and clinical evidence