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The harvested cells were resuspended in 20 mM Tris-HCl, pH 7.4, 300 mM NaCl, 10% glycerol, 1 mM DTT, and 10 mM imidazole and lysed by sonication

223 : e202309140

PMID: 40929057 Sci Rep Impact of prenatal Di(2-ethylhexyl) phthalate exposure on pubertal development in female offspring rats: A focus on ER-Mediated IGF-1/NKB crosstalk in the hypothalamus