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El factor intrnseco (FI) es una glicoprotena secretada por las clulas parietales de la mucosa gstrica que tiene la funcin de unirse a la vitamina B12 una vez sta se ha desprendido de las protenas R, otorgndole resistencia a las proteasas gstricas y pancreticas, formando un complejo B12+factor intrnseco que se conoce como complejo de Castle

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If it does not dissolve after 5 minutes of gentle swirling, possible causes include: degraded peptide (check storage history and expiration status ), contaminated water (use a fresh vial of bacteriostatic water), or water temperature (cold water from the refrigerator dissolves peptides more slowly than room temperature water)

Notably, it is difficult to calculate an accurate FDR using the entrapment strategy given that it is unlikely to know the exact number of phosphopeptides in the human sub-library that is not present in the sample

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