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After parasite pellet resuspension in SDS lysis buffer (50 mm Tris-HCl pH 8, 10 mm EDTA pH 8, 1% SDS), protein quantification was performed with a bicinchoninic acid assay kit (Abcam) and for each condition, 20 g of total proteins were separated on a 12% SDS-PAGE run for 20 min at 100 V, stained with colloidal blue (Thermo Fisher Scientific)

Purification was performed using the BacMax 100 kit (Macherey-Nagel, Duren, Germany) and transfected into T-Rex-293 cells using the Effectene transfection kit (Qiagen) in T25 tissue culture flasks

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Although researchers believe that the cytokines are critical for the crosstalk between ferroptosis cells and immune cells, the mechanism remains unclear

How IV Therapy Is Being Used IV therapy delivers vitamins, minerals, antioxidants, and fluids directly into the bloodstream, allowing for faster absorption than oral supplements