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After three times washing with PBS, cells were trypsinized and resuspended in PBS for flow cytometric analysis with a BD FACSCalibur Flow Cytometer

Lyon: Interpol

In aged or photodamaged skin, GHK-Cu treatment (1-5 M) suppresses elevated MMP-1 (collagenase-1) expression by approximately 40-60%, as documented through quantitative PCR and Western blot analyses

Next, a Bertin 24 homogenizer (Bertin Technologies) was run at 5,000 rpm (2 cycles of 45 s with a pause of 5 s in between) to lyse the microbial cells