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The cells were then incubated with primary antibodies directed against -H2AX (Sigma, 05-636, dilution 1:100), CD31 (Abcam, ab215912, dilution 1:50), or -SMA (Abcam, ab5694, dilution 1:1000) overnight at 4 C on a shaker

Wan X, Song L, Pan W, et al

Bull Natl Res Cent 44 , 18 (2020)

These volumes were subjected to symmetric search utility in CryoSPARC to determine twist and rise parameters

Since the majority of physicians using the combination treatment DMPS+CaEDTA or DMPS+ZnDTPA, do not collect baseline urines for comparison, we could select few pairs of pre and post chelation samples