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Comparative protein modelling by satisfaction of spatial restraints

Sies H (1999)

For example, one of the possible mechanisms of folding of the deeply knotted proteins relies on the ribosome pushing the chain through the attached loop

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Reconstitute using sterile bacteriostatic water or an appropriate laboratory buffer Add solvent slowly along the vial wall to minimize foaming during dissolution Avoid vigorous agitation, shaking, or vortexing of the peptide solution Gently swirl or invert the vial until the peptide is fully dissolved Store reconstituted solutions at 3646 F (28 C) during short term use Prepare aliquots where appropriate to reduce repeated freezethaw exposure Careful reconstitution practices help maintain peptide stability and support reliable experimental conditions