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Materials And Methods Antibodies and chemicals The following primary antibodies were purchased from Cell Signaling Technology (MA, USA) and used at the indicated dilutions for western blot analysis: CD44 (#3570S, 1:1000), CD133 (#64326S, 1:1000), PBEF/NAMPT (#86634S, 1:1000), lamin B1 (#12586S, 1:1 000), hexokinase II (#2867S, 1:1000), ATP-Citrate Lyase (#13390S, 1:1000), HMGCS1 (#36877S, 1:1000), and Cdc2/CDK1 (#77055, 1:1000)

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Cayman Chemical) was added to the supernatant fraction to limit oxidation and/or isomerization of reactive compounds 60

Similar content being viewed by others Introduction The average age of the worlds population is increasing year on year due to people living longer and a decline in the birth rate (Beard et al 2016)

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