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Subsequently, the slices were incubated with the primary antibody: Anti-Vimentin (1:400), Anti-Nestin (1:200), Anti-GFAP (1:200) and Anti-3F3-FMA (1:200), anti-MDA (1:100) throughout the overnight

MiR-33a and MiR-33b promote atherosclerosis by impairing cholesterol efflux and promoting inflammatory M1 macrophage conversion (78-80).Other microRNAs including MiR-223 and MiR-93 exhibit atheroprotective effects by increasing cholesterol efflux and conversion to the anti-inflammatory M2 macrophage phenotype (76,81-83)

2006, June: 8th Annual Family Caf/Family Collaboration Advocacy Friendship Empowerment Conference, presenter on Planning and Preparing for Obtaining Employment to Gain More Independence

Gilliams-Francis KL, Quaye AA, Naegele JR

We then used ethanol precipitation to increase DNA concentration in each sample through addition of 19 L 3 M Sodium acetate (pH 5.2) and 475 L cold ethanol