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Lipid measurements using LCMS For lipid extraction, samples were mixed with 20 C isopropanol (150 l per 5 l serum and 40 l per mg tissue), vortexed and immediately centrifuged at 16,000 g for 10 min at 4 C 15

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We then constructed luciferase reporters fused with the SLC7A11 -3-UTR (WT) or mutated SLC7A11 -3-UTR (MT) to test whether miR-30e-5p directly targets the SLC7A11 mRNA (Figure 2E)

doi: 10.3389/fpls.2019.01743 Received 25 August 2019 Accepted 11 December 2019 Published 24 January 2020 Volume 10 - 2019 Edited by Veronica Graciela Maurino, University of Bonn, Germany Reviewed by Frantisek Baluska, University of Bonn, Germany