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After 24 hours from the first trial, a second trial is performed by placing the animal in the same container, but for 5 minutes

L-glutamine: Supports gut, immune function, and recovery

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While safety monitoring was comprehensive, the trials were not specifically designed to detect rare adverse effects that might occur at rates lower than 1-2%, which would require much larger participant numbers

Both univariate and multivariate Cox proportional hazards regression analyses were performed to assess the prognostic value of the drug-related signature (DRS) scores, adjusting for potential confounders