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In deeply anaesthetised rats (placed in ventral position) the tail was transected with a surgical scalpel 3 cm from the tip (and submerged into a tube with 15 ml of saline at room temperature in vertical position) and the duration and amount of bleeding were measured to evaluate the hemostatic effect of the agents or saline administration

10 The standard supplement dose is 2 g taken twice daily (4 g total) for at least 3 months

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1b), a scenario that closely resembled the clinical setting for most patients at diagnosis

Moreover, EA treatment improved ovarian morphology, reduced theca layer thickness, enhanced the oocyte layer, and improved the quality of antral and preovulatory follicles ( Figure 1 )