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For protein expression, auto-inducing ZYM medium (per liter: tryptone 10 g, yeast extract 5 g, glycerol 5 g, glucose 0.5 g, l-arabinose 2 g, Na 2 HPO 4 25 mM, KH 2 PO 4 25 mM, NH 4 Cl 50 mM, Na 2 SO 4 5 mM, MgSO 4 2 mM, and trace elements including 0.05 mM FeCl 3 , 0.02 mM CaCl 2 , 0.01 mM MnCl 2 , 0.01 mM ZnSO 4 , and 0.002 mM each of CoCl 2 , NiCl 2 , Na 2 Mo 4 , Na 2 SeO 3 , and H 3 BO 3 ) was used [35], and the strains were allowed to auto-induced at 30 C for 1216 h

doi: 10.1002/pep2.24202 Summary Keywords antimicrobial peptides, biotic and abiotic stresses, salinity, drought, pathogenic fungi, plant diseases, insect pests, transgenic plants Citation Ha-Tran DM and Huang C-C (2025) Peptides from plants and microorganisms: a defense strategy for biotic and abiotic stresses

PMID: 217 HangSPaikDYaoLKimETrinathJLuJet al

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And the amazing thing is all three of those things need to be present for the autoimmune condition to manifest