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J Wuhan Institute Phys Educ

A 2:1 ratio of Tyr to Cu(II) was found to give the best results

Research focuses on its impact on metabolism and cellular energy balance

Frozen tissues were disrupted in 50:50 cold methanol/0.1 M hydrogen chloride and homogenized at high speed by shaking with stainless-steel beads into a TissueLyser LT (QIAGEN)